Humanized Anti-RNF43 Recombinant Antibody (TAB-328CT)
CAT#: TAB-328CT
This product is a recombinant humanized antibody that recognizes RNF43. The antibody was purified by affinity chromatography. It can be used for WB, ELISA, FC, IHC, IP and other applications.
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Anti-Human RNF43 Recombinant Antibody (TAB-328CT) in SDS-PAGE
Lane M: Marker
Lane 1: TAB-328CT in non-reduced condition.
Lane 2: TAB-328CT in reduced condition.
HPLC
Figure 2 Anti-Human RNF43 Recombinant Antibody (TAB-328CT) in HPLC
HPLC analysis of TAB-328CT shows the purity is>94%.
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Normal Tissue
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_7_5_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8079/23746_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108375-RNF43
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Specifications
- Immunogen
- Recombinant human RNF43-Fc protein
- Host Species
- Humanized
- Derivation
- Mouse hybridoma cell line
- Type
- Humanized IgG1, κ
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Western Blot; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Immunohistochemistry; Immunoprecipitation
- Related Disease
- Colorectal cancer,lung cancer.
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Reliable in Signal Pathway Analysis
We've used this antibody extensively in analyzing Wnt signaling pathways. Its high specificity and affinity make it reliable for detecting RNF43 interactions and modifications, providing valuable data for our experiments.
Excellent for Western Blotting
We've integrated this antibody into our Western blotting protocols, where it consistently delivers clear, concise results. It's been pivotal for determining RNF43 expression levels under various experimental conditions.
Adaptable for Various Applications
Its adaptability for use in WB, IP, and IF demonstrates its versatility, which is beneficial for labs requiring multi-faceted analysis tools within their experimental setups.
Q&As
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What is the primary target of the Anti-RNF43 Recombinant Antibody?
A: The primary target is the RING finger protein 43 (RNF43), a transmembrane E3 ubiquitin ligase involved in regulating Wnt signaling pathways.
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In which research applications is this antibody commonly used?
A: This antibody is commonly used in Western blot (WB), immunoprecipitation (IP), and immunofluorescence (IF) for studying protein expressions and interactions.
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What are the specific applications for this recombinant antibody?
A: Beyond WB, IP, and IF, it can be used in enzyme-linked immunosorbent assays (ELISA) to quantify protein levels and potentially in flow cytometry (FC) for cellular analysis.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-328CT, RRID: AB_3111913)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "RNF43"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-327CT | Mouse Anti-RNF43 Recombinant Antibody (TAB-327CT) | WB, ELISA, FC, IHC, IP | Mouse IgG |
| TAB-327CT-S(P) | Mouse Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-327CT-S(P)) | WB, ELISA, FC, IHC, IP | Mouse scFv |
| TAB-327CT-F(E) | Mouse Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-327CT-F(E)) | WB, ELISA, FC, IHC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-328CT-S(P) | Humanized Anti-RNF43 Recombinant Antibody; scFv Fragment (TAB-328CT-S(P)) | WB, ELISA, FC, IHC, IP | Humanized scFv |
| TAB-328CT-F(E) | Humanized Anti-RNF43 Recombinant Antibody; Fab Fragment (TAB-328CT-F(E)) | WB, ELISA, FC, IHC, IP | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN1185 | PE-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer | FCM, IHC | |
| MHC-CN1186 | APC-A*02:01/Human RNF43 (ALWPWLLMAT) MHC Tetramer | FCM, IHC | |
| MHC-YF705 | A*02:01/Human RNF43 (ALWPWLLMA) MHC Pentamer | FCM | |
| MHC-YF706 | A*02:01/Human RNF43 (ALWPWLLMAT) MHC Pentamer | FCM | |
| MHC-YF707 | A*24:02/Human RNF43 (722Y) (NYQPVWLCL) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC181 | Recombinant Anti-RNF43 Vesicular Antibody, EV Displayed (VS-0425-YC181) | ELISA, FC, Cell-uptake |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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